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Differential expression of genes in follicular cells of swines R. Bras. Zootec.
Miranda-Furtado,Cristiana Libardi; Silva,Priscila Vendramini; Guimarães,Marta Fonseca Martins; Serão,Nicola Vergara Lopes; Guimarães,José Domingos; Guimarães,Simone Eliza Facioni.
The main purpose of the present study was to identify for candidate genes related to ovulation in swines. To do so, it was investigated in ovarian follicular cells through quantitative real-time PCR the differential expression of the following genes: steroidogenic acute regulator (STAR), GATA-binding protein 4 (GATA), prostaglandin F2α (PGF2α), progesterone receptor (P4R), follicle-stimulating hormone receptor (FSHR), and cytochrome P450 aromatase (CYP19). These genes encode hormone receptors (FSHR and P4R), hormone (PGF2α), steroidogenic proteins (STAR and CYP19) and transcription factor (GATA). Folicular cells were collected from sows with high and low number of piglets/litters during the follicular phase of the estrus cycle. There was difference in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candidate gene; Litter size; Ovulation rate; Pig production; Quantitative real-time PCR.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010000500012
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Genetic diversity of captive spotted paca (Agouti paca) from south east Brazil assessed by the RAPD-PCR technique R. Bras. Zootec.
Antunes,Karine Vieira; Machado,Théa Mírian Medeiros; Serão,Nicola Vergara Lopes; Guimarães,Simone Eliza Facione; Paiva,Samuel Rezende.
The genetic diversity was analyzed among spotted paca (Agouti paca) from three commercial flocks located in Brazil. As the genome of this species is unknown, the RAPD-PCR technique was used. Ten primers generated sixty polymorphic bands. The among and within population genetic variability estimated by analysis of molecular variance (AMOVA) was 12.55 and 87.45%, respectively. The shortest Nei distance value was 11.76% among the Carangola (CG) and São Francisco do Glória (SF) populations. This value can be explained by the exchange of reproduction males and females between the two geographically close breeding sites. The analysis of principal components showed well defined and structured groups aggregating animals according their population of origin, with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Animal conservation; Animal genetic resources; Biodiversity; Genetic distance; Wild animals.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010000200006
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Lisina digestível para leitoas em fase de crescimento Ciência Rural
Rocha,Gabriel Cipriano; Donzele,Juarez Lopes; Donzele,Rita Flavia Miranda de Oliveira; Silva,Francisco Carlos de Oliveira da; Kiefer,Charles; Alebrante,Leandro; Paulino,Pedro Veiga Rodrigues; Serão,Nicola Vergara Lopes.
Oitenta leitoas (24,2±1,52kg) com alto potencial genético para deposição de carne na carcaça foram distribuídas em experimento de blocos ao acaso para avaliar cinco níveis de lisina digestível (Ld) (9, 10, 11, 12 e 13g kg-1) durante a fase de crescimento (63 a 103 dias de idade). Os animais foram alojados em pares e alimentados à vontade. No início e ao final do período experimental, as leitoas foram pesadas e submetidas à análise de ultrassom para avaliação da área de olho de lombo (AOL) e espessura de toucinho (ET). Os níveis de Ld proporcionaram aumento linear (P<0,05) do ganho de peso diário (GPD) e, apesar dessa variação, não houve aumento no valor absoluto do GPD a partir do nível de 12g kg-1 de Ld. A conversão alimentar (CA) reduziu (P<0,01)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carcaça; Exigência nutricional; Desempenho; Suínos.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000500019
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Nutritional plans of digestible lysine for growing-finishing gilts R. Bras. Zootec.
Rocha,Gabriel Cipriano; Donzele,Juarez Lopes; Silva,Francisco Carlos de Oliveira; Donzele,Rita Flávia Miranda de Oliveira; Kiefer,Charles; Alebrante,Leandro; Paulino,Pedro Veiga Rodrigues; Serão,Nicola Vergara Lopes.
This experiment was conducted to evaluate nutritional plans of digestible lysine (DLys) for growing-finishing gilts. Eighty gilts with 63 days of age and an initial weight of 24.2±1.52 kg were distributed in a completely randomized block design, with five nutritional plans of DLys (9-8-7, 10-9-8, 11-10-9, 12-11-10, and 13-12-11 g/kg, from 63 to 103, 104 to 133, and 134 to 153 days of age, respectively) and eight replicates. Pigs were housed in pairs and fed their respective diets ad libitum throughout the experimental period (90 days). To monitor the animal development along the experiment at 103 and 133 days, gilts were weighed and subjected to analysis of ultrasound for evaluation of loin depth (longissimus dorsi) and backfat thickness. At the end of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acid; Carcass; Performance; Phases; Swine.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014000900457
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